T5 DNA Polymerase

In the Public Domain since

29 Jul 2006



The present invention discloses a recombinant DNA molecule having a structural gene encoding a processive, thioredoxin-independent DNA polymerase that is substantially reduced in processive 3'-to-5' DNA exonuclease activity, a promoter, and an origin of replication. A method for producing this enzyme is also disclosed, as is the protein produced by this process. This invention is exemplified by expression of exonuclease- T5 DNA polymerase in E. coli.


Enzymes > DNA Polymerases

Related Inventors

Chatterjee, Deb K.


US Patent No 5,716,819

Patent Title: Cloning And Expression Of T5 Dna Polymerase Reduced In 3'-to-5' Exonuclease Activity

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