T5 DNA Polymerase
In the Public Domain since
29 Jul 2006
Description
Abstract
The present invention discloses a recombinant DNA molecule having a structural gene encoding a processive, thioredoxin-independent DNA polymerase that is substantially reduced in processive 3'-to-5' DNA exonuclease activity, a promoter, and an origin of replication. A method for producing this enzyme is also disclosed, as is the protein produced by this process. This invention is exemplified by expression of exonuclease- T5 DNA polymerase in E. coli.
Category
Enzymes > DNA Polymerases
Related Inventors
Chatterjee, Deb K.
Source
US Patent No 5,716,819
Patent Title: Cloning And Expression Of T5 Dna Polymerase Reduced In 3'-to-5' Exonuclease Activity