T4 DNA Polymerase

In the Public Domain since

18 Sep 2006



The DNA sequence encoding T4 DNA polymerase (gene 43 of T4 phase) has been successfully cloned, thereby providing a convenient source of this enzyme. The method involves the production of a gene 43 NH2-terminus fragment absent its promoter. The latter is ligated to a fragment containing the remaining COOH-terminus sequence but lacking base pairs beyond this terminus which heretofore caused destabilization effects in cloning. The full sequence of the DNA sequence has been elucidated. Corresponding cloning methods are disclosed.


Enzymes > DNA Polymerases

Related Inventors

Lin, Tsung-Chung ; Konigsberg, William ; Spicer, Eleanor and Rush, John E.


US Patent No 4,935,361

Patent Title: Cloning And Expression Of T4 Dna Polymerase

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