T4 DNA Polymerase
In the Public Domain since
18 Sep 2006
Description
Abstract
The DNA sequence encoding T4 DNA polymerase (gene 43 of T4 phase) has been successfully cloned, thereby providing a convenient source of this enzyme. The method involves the production of a gene 43 NH2-terminus fragment absent its promoter. The latter is ligated to a fragment containing the remaining COOH-terminus sequence but lacking base pairs beyond this terminus which heretofore caused destabilization effects in cloning. The full sequence of the DNA sequence has been elucidated. Corresponding cloning methods are disclosed.
Category
Enzymes > DNA Polymerases
Related Inventors
Lin, Tsung-Chung ; Konigsberg, William ; Spicer, Eleanor and Rush, John E.
Source
US Patent No 4,935,361
Patent Title: Cloning And Expression Of T4 Dna Polymerase